ABSTRACT
Objective To study apoptosis and antigen presentation changes of monocytes in HBV-related acute-on-chronic liver failure (ACLF) patients under immune dysfunction state.Methods Peripheral blood samples of 26 HBV-related ACLF patients (ACLF group),20 active chronic hepatitis B patients (CHB group) and 18 healthy individuals (control group) were collected.The changes of apoptosis and proliferation (Ki67) in monocytes and the expression of surface markers including human leukocyte antigen (HLA)-DR and B7 molecules (CD86) of monocytes were analyzed by flow cytometry. Results The percentage of Annexin V expressed monocytes of ACLF group (64％) was significantly higher than that of CHB group (28％) and control group (20％),and the difference was statistically significant (x2 value was 11.75 and 27.23 ; both P＜0.01),which indicated that monocytes apoptosis increased.The Ki67 expression in monocytes of ACLF group was lower than that of CHB group and control group,and the difference was statistically significant (x2 value was 4.71 and 4.83; both P＜ 0.05),which indicated that activated monocytes reduced. The mean fluorescence intensity (MFI) of HLA-DR and CD86 of monocytes in ACLF group was 22.85 and 11.63,which was significantly lower than that of CHB group and control group,indicating the antigen presentation ability of monocytes injured. The percentage of Annexin Ⅴ positive monocytes in survivals (62 ％ ) was significantly higher than that of dead patients (46 ％ ) in ACLF group.Conclusion In HBV-related ACLF patients under immune dysfunction state,the apoptosis of peripheral blood monocytes increased,and the quantity of activated cells reduced,resulting in the decline of the antigen presentation ability of monocytes.
ABSTRACT
[Objective]To observe the clinical treatment of calcaneus fracture with poking reduction and artificial bone grafting technique.[Method]From 1999.6 to 2005.5,60 cases with recent calcaneus fracture,including 46 feet in 38 males and 24 feet in 22 females with falling injure;aged from 20 to 65,were equally divided into two groups,including the observed group treated with poking reduction and artificial bone grafting and the control group with steel needle pry-poking reposition.[Result]During followed up for 6-60 months,average 20.7months,it was notable different in Maryland foot score,and the same in Bhler′,Gissane′ and Perie′ angle in X-ray in post-operation and pre-operative between observed group and the control.[Conclusion]It showed us that the technique of poking reduction and artificial bone grafting was liable to operate and could provide exactly efficiency.